Quantitative polymerase chain reaction (qPCR) is a molecular technique that has reshaped the landscape of biological research and medicine due to its high sensitivity and specificity. qPCR, also known ...

RT-qPCR stands for quantitative reverse transcription polymerase chain reaction, the “RT” not to be mistaken for “real time”. 2 Unlike qPCR that uses a DNA template, the starting material for RT-qPCR ...