A mouse brain slice (5 days old, 300 μm thick) was cleared with SeeDB-Live. The medium was exchanged from artificial cerebrospinal fluid (ACSF) to SeeDB-Live/ACSF, and within one hour of immersion, ...

Time-lapse two-photon imaging of the calcium indicator, GCaMP6f, in an acute slice of a 11-day-old mouse olfactory bulb (depth, 150 μm). GCaMP6f was expressed in mitral cells. The medium was exchanged ...