The composition of ancient oral microbiomes has recently become accessible owing to advanced biomolecular methods such as metagenomics and metaproteomics, but the utility of metaproteomics for such ...

To minimize environmental contamination, each dental calculus sample was UV-treated for 15 min on each side, soaked in 2 ml of 5% bleach for 3 min, rinsed in 90% ethanol for 1 min, and dried at room ...